A universal and rapid protocol for protein extraction from recalcitrant plant tissues for proteomic analysis.
نویسندگان
چکیده
A simple and universally applicable protocol for extracting high-quality proteins from recalcitrant plant tissues is described. We have used the protocol with no modification, for a wide range of leaves and fruits. In all cases, this protocol allows to obtain good electrophoretic separation of proteins. As the protocol is rapid, universal, and compatible with silver staining, it could be used for routine protein extraction from recalcitrant plant tissues for proteomic analysis.
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ورودعنوان ژورنال:
- Electrophoresis
دوره 27 13 شماره
صفحات -
تاریخ انتشار 2006